PolyJet™ In Vitro DNA Transfection Reagent

Description:
Based on our proprietary polymer synthesis technology, PolyJet™ DNA In Vitro Transfection Reagent is formulated as a biodegradable polymer-based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO, and a broad range of hard-to-transfect mammalian cells. The PolyJet™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form the transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of the polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24-well plates or ~333 transfections in 6-well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.

Figure 1. A Cartoon Showing Biodegradation of PolyJet™ DNA Transfection Reagent After Endocytosis of Transfection Complex

Features:
– Bio-degradable after endocytosis
– Exceptionally high titers of virus production
– Equally good for very long DNAs (>89 kb)
– Equally good for both single DNA transfection and multi DNA co-transfection
– High levels of recombinant protein production
– Simple & robust transfection procedure
– Very affordable

Storage Condition:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Broad Transfection Spectrum for Mammalian Cell Types

Examples Showing Transfection Efficiency of PolyJet™ DNA In Vitro Transfection Reagent on Commonly Used Cells

Transfection efficiency comparison of PolyJet™ vs. lipofectamine Plus on Chinese Hamster Ovary (CHO) cells. HA tagged beta-tubulin cDNA was delivered into CHO cells with PolyJet™ (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). The above picture was provided by Dr. Shang Yin of University of Texas at Houston Medical School as courtesy

A comparison showing the transfection efficiency of PolyJet™ reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. HEK-293FT cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection

A comparison showing transfection efficiency of PolyJet™ reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post-transfection

Transfection efficiency comparison of PolyJet™ vs. Fugene HD on MDCK cells. A plain GFP DNA was transduced into MDCK cells with PolyJet™ (left panel) and Fugene HD (right panel) reagents, respectively per the manufacturers’ protocols. GFP and DAPI staining were visualized under fluorescence microscopy 48 hours post-transfection. The above comparison data and pictures were completed and provided by Dr. Ge Zhou of NYU Medical Center as courtesy

A comparison showing the transfection efficiency of PolyJet™ reagent vs. a leading product, Lipofectamine 2000 on MDCK cells. MDCK cells are notoriously hard to transfect. With proprietary “Shaved Cell Transfection” protocol, PolyJet™ (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel), around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection

A comparison showing the transfection efficiency of PolyJet™ reagent vs. a leading product, Fugene HD on LNCap cells. LNCap cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Fugene HD (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection

An image showing the exceptional transfection efficiency of PolyJet™ reagent on Human embryonic stem cells (hESCs). The hESCs grown in E8 medium on Geltrexvs (left panel, DIC imaging) was transfected with pEF1α-GFP. The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope 24 hours post-transfection. The above pictures were provided by Dr. Marina Pryzhkova of Johns Hopkins University as a courtesy

Neuro2A cells transfected with pEGFP-C1 plasmid using PolyJet™ In Vitro DNA Transfection Reagent. The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection

Comparison of cytotoxicity of PolyJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium, followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post-transfection

Data Sheet and Protocols
– A General Protocol for Transfecting Mammalian Cells
– A Short Protocol for Advanced Users 
– A Protocol for Lentivirus Packaging in HEK293T Cells  
– A Protocol for AAV Packaging in HEK293T Cells 
– A Protocol for Adenovirus Packaging in HEK293A Cells 
– A Protocol for Transfecting Suspension 293 and CHO Cells
– Technical Note & Transfection Tips