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GenMute™ siRNA Transfection Reagent for MEFs
Description:
GenMute™ siRNA Transfection Reagent for MEFs is specifically optimized for efficient siRNA delivery into mouse embryonic fibroblasts (MEFs). MEFs are commonly used as feeder cells to support the growth and maintenance of undifferentiated mouse or human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. Derived from mouse embryos and typically used at early passages, MEFs provide essential growth factors and extracellular support. Prior to use as feeder layers, MEFs are growth-arrested by irradiation or mitomycin C treatment to prevent further cell division while preserving their supportive functions.
Size & content:
– GenMute™ Reagent, 1.0 mL, sufficient for ~833 reactions based on transfecting 20 pmol siRNA or miRNA mimics in a 24-well plate
– GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution
Refer to the following optimal transfection conditions for maximal silencing on MEFs. GenMute™ reagent, 1.0 mL, is sufficient for ~833 transfections in 24-well plates or ~416 transfections in 6-well plates.
| Summary of Optimal Transfection Conditions: Cell culture medium Confluency of the cell on the day of transfection Optimal siRNA concentration Diluent for siRNA and GenMute™ Reagent Incubation Time to Form GenMute™/siRNA Complex Maximal Silencing Timeline Reporter Gene Silencing Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS ~50% 40 nM 1xGenMute™ Transfection Buffer ~15 min at RT 72 hours Renilla Luciferase 90% |
Storage: Store at 4 °C. If stored properly, the product is stable for 24 months or longer
Transfection Protocol 
CITATIONS
| Shipping Weight | 6 lbs |
|---|---|
| Unit Size | 5 x 1.0 mL, 1.0 mL, 0.5 mL, 0.1 mL |
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Partnership Opportunities
We offer OEM manufacturing services for our transfection reagents to qualified life science reagent companies for resale under their own brand names. Interested partners are encouraged to contact us for additional information regarding OEM collaboration opportunities.
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