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A Proven, Streamlined Workflow for Stable Cell Line Generation
A standardized lentivirus-based workflow for stable cell line generation, enabling efficient integration and consistent, reproducible expression across diverse cells
Vector Design, Cloning & Production
Design & construct a vector for transgene overexpression or gene silencing (via shRNA, shRNAmir, gRNA, miRNA, or TuD RNA), incorporating a selection marker, followed by viral particle production for efficient delivery
WEEK 1~2
Cell Transduction & Selection
Infect target cells, HEK293, CHO. HeLa or custom, with lentivirus under optimized conditions, followed by antibiotic selection to enrich cells with stable genomic integration
WEEK 3~4
Clonal Isolation & Expansion
Isolate single-cell–derived clones (e.g., limiting dilution or sorting) and expand them to establish uniform cell populations
WEEK 5~6
Screening, Validation & QCs
Assess expression levels via RT-qPCR, Western Blot, verify functional performance, and confirm long-term stability before generating a master cell bank
WEEK 7
Why SignaGen?
Reasons customers trust us for stable cell generation
Rigorous Quality Validation
Technical Benchmarks
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Discuss your stable cell line project with our PhD scientists and request a quote
We price-match competitors & provide an additional 10% discount
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