High-titer Custom Lentivirus Production Full Services


Custom Vector Design and High-Titer Lentivirus Production Aligned with Your Research Requirements

Comprehensive Full-Service Lentivirus Production Solutions

Scalable lentivirus production solutions encompassing vector design, optional gene synthesis, lentivirus packaging, purification, and quality control

Gene Overexpression or Lentiviral Homology-Directed Repair

Gene Silencing via Lentiviral shRNA, shRNAmir, miRNA, gRNA & TuD RNA

“Concept-to-Delivery” Workflow

SignaGen provides a streamlined workflow that ensures rapid turnaround while maintaining the highest standards of scientific rigor

Lentivector Design & Gene Synthesis

Software-based lentivector design for transgene or shRNA, shRNAmir, miRNA, gRNA, or TuD RNA overexpression

WEEK 1

Lentivector Cloning & Verification

GAC or RE cloning followed by verification via restriction digestion & WPS via NanoPore platform

WEEK 2

Lentivirus Packaging & Harvest

Lentivirus packaging via LentiMax™ system in HEK293T cells, followed by lentivirus harvest

WEEK 3

Lentivirus Purification & QCs

Lentivirus purification by sucrose ultracentrifugation, followed by RT-qPCR titration

WEEK 4

Why SignaGen?

Reasons customers trust us for lentivirus production


Proven Lentivirus Expertise

10+ years of excellence | 7,000+ scientific citations | 110+ off-the-shelf lentiviruses available

Cost-Effective & Affordable

We price-match competitors & provide an additional 10% discount

Customizable & Scalable

Final deliverables from 250 µL to 5000 µL at >10⁹ TU/mL for in vitro and in vivo applications

Fast Turnaround

~4-week lentivirus production full service | ~2-week lentivirus packaging service

Extensive Options for Promoter & Pseudotyped Envelopes

Constitutively active or tissue-specific gene expression enabled by our extensive promoter and lentiviral pseudotyped envelope library

600+ empty lentiviral transfer vectors featuring diverse promoters, introns, and WPRE configurations

Four naturally occurring glycoproteins available as pseudotyped envelope options

Custom-engineered lentiviral envelope synthesis is available when off-the-shelf options do not meet your specific requirements

Lentivirus design-related IP belongs to customer, who receives the final lentivirus with the cloned transfer construct

Rigorous Quality Validation

RT-qPCR / p24 ELISA Titer Determination

Precise quantification of viral genomes and p24 protein for highly reproducible dosing

Purity & Identity

Silver-stained SDS-PAGE to verify capsid protein integrity

Safety Testing

LAL assay for Endotoxins (<0.1 EU/mL), mycoplasma screening, and RCL assay

Technical Benchmarks

  • Titer: >10⁹ TU/mL
  • Purity: In vivo grade Ok for direct injection
  • Sterility: Ensure no bacterial/fungal growth
  • Turnaround: ~4 weeks

We Offer Fully Customizable Lentivirus Production Services


We price-match competitors & provide an additional 10% discount

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