GenJet™ Plus In Vivo DNA Transfection Reagent

Description
GenJet™ Plus In Vivo DNA Transfection Reagent is an advanced non-viral gene delivery system derived from the original GenJet™ DNA Transfection Reagent and further optimized through the incorporation of multiple proprietary cationic and functional peptides. These modifications significantly increase the density of DNA-binding and condensing moieties, resulting in enhanced plasmid DNA complexation, stability, and in vivo delivery efficiency. In addition, a covalently linked shielding peptide minimizes nonspecific interactions with serum components and other biological barriers, thereby improving systemic circulation and transfection performance in vivo. GenJet™ Plus has been demonstrated to mediate robust transgene expression following systemic administration, with high levels of Renilla luciferase activity detected in multiple organs, including the lung, liver, spleen, and kidney.

Storage Conditions
Store at 2–8°C. Under the recommended storage conditions, GenJet™ Plus DNA In Vivo Transfection Reagent remains stable for a minimum of 24 months.

Performance Evaluation
The in vivo transfection efficiency of GenJet™ Plus reagent was evaluated in mice using a plasmid encoding Renilla luciferase as a reporter gene. Following systemic administration, transgene expression was quantified in multiple organs and compared with alternative delivery approaches. GenJet™ Plus consistently demonstrated efficient plasmid DNA delivery and strong transgene expression across a broad range of tissues, confirming its suitability for systemic in vivo gene transfer applications.

Transfection Protocol