Service Quote Request
Please fill out the form below with your detailed inquiry.
-
PHONE: 1-866-918-6812
High-Precision Gene Editing
Supporting the CRISPR/Cas9 revolution, SignaGen offers custom-engineered viral vectors for efficient and reproducible genome editing applications. Our scientific services team provides end-to-end technical support, including guide RNA (gRNA) design, vector construction, and molecular cloning, to ensure accurate and high-efficiency delivery of genome-editing components into both standard and difficult-to-transduce cell types.
Adenovirus (AdV), adeno-associated virus (AAV), and lentivirus (LV) represent the principal viral platforms for delivering gene-editing systems such as CRISPR/Cas9, base editors, and prime editors. Each vector system addresses distinct experimental and logistical requirements, including payload capacity, transduction efficiency, and expression kinetics. The table below summarizes the key features and advantages of adenoviral, AAV, and lentiviral vectors for genome-editing applications.
| Vector | Payload Capacity | Expression Duration | Best Gene Editing Application |
| Adenovirus | High (8 kb) | Transient (Episomal) | “Hit-and-Run” editing: Delivering large editors (Base/Prime) without permanent integration to minimize off-target cuts |
| AAV | Low (4.7kb) | Long-term (Non-integrating) | In vivo therapy: Precision editing in specific tissues (brain, eye, liver) using smaller Cas9 variants (e.g. SaCas9) |
| Lentivirus | High (8 kb) | Permanent (Integrating) | CRISPR Screens: Creating stable cell lines that constitutively express Cas9 for genome-wide knockout libraries |
Off-the-shelf control viral vectors for high-precision gene editing
| SKU # | Viral Vector |
|---|---|
| SL101136 | AAV1-CMV-SaCas9-U6-gRNA(SCRM) |
| SL101137 | AAV9-CMV-saCas9-U6-gRNA(SCRM) |
| SL101404 | AAV9-CMV-hSpCas9 |
| SL101465 | AAV8-CMV-saCas9-U6-gRNA(SCRM) |
| SL116041 | AAV9-CMV-SaCas9 |
| SL116042 | AAV9-Syn-SaCas9 |
| SL116043 | AAV1-CMV-SaCas9 |
| SL116044 | AAV1-Syn-SaCas9 |
| SL116046 | AAV1-Mecp2-SpCas9(D10A) |
| SL116047 | AAV2-Mecp2-SpCas9(D10A) |
| SL116048 | AAV9-Mecp2-SpCas9(D10A) |
| SL101404 | AAV9-miniCMV-SpCas9 |
| SL116408 | AAV9-Syn-SaCas9 |
| SL100307 | LV-EF1α-SpCas9 |
| SL100310 | LV-CMV-SpCas9 |
| SL101106 | Ad-CMV-SpCas9 |
| SL101107 | Ad-CMV-Cas9(D10A)-T2A-DasherGFP |
| SL101713 | Ad-CMV-SaCas9 |
| SL101714 | Ad-CMV-SaCas9-CMV-GFP |
| SL101751 | Ad-CMV-3xFLAG-SpCas9-P2A-PuroR |
Custom viral vector production services for high-precision gene editing
Still can’t find the viral vectors you need for CRISPR based gene editing in our off-the-shelf inventory? We offer custom viral vector production tailored for gene editing applications. Let SignaGen build your custom viral vectors, so you can stay focused on your science while we handle the vector design and production.
gRNA AAV Production Full Service — SignaGen provides full-service recombinant gRNA AAV production, encompassing gRNA design, oligonucleotide synthesis, and vector cloning through delivery of in vivo–grade viral preparations at high and ultra-high titers. Due to the limited packaging capacity of AAV vectors, the use of SaCas9 is recommended for efficient genome-editing applications. Production scales are available up to 100 mL, with titers exceeding 1 × 10¹³ VG/mL.
gRNA Lentivirus Production Full Service — SignaGen provides comprehensive, end-to-end gRNA lentivirus production services, encompassing gene synthesis, vector construction, and delivery of in vivo–grade lentiviral vectors. Both SaCas9 and SpCas9 systems can be incorporated into the lentiviral vector to support a wide range of genome-editing applications. High-titer and ultra-high-titer preparations are available at production scales of up to 5.0 mL, with achievable titers exceeding 1 × 10⁹ TU/mL.
gRNA Adenovirus Production Full Service — SignaGen offers comprehensive, end-to-end recombinant gRNA adenovirus production services, encompassing gene synthesis, vector construction, and delivery of in vivo–grade adenoviral vectors. Both SaCas9 and SpCas9 systems can be incorporated into the adenoviral vector for genome-editing applications. High-titer and ultra-high-titer preparations are available at production scales of up to 5 mL, with achievable titers exceeding 5 × 10¹⁰ PFU/mL.
Need custom viral vectors for efficient CRISPR-mediated gene editing?
We offer end-to-end solutions, including vector design, plasmid construction, viral packaging, purification, and quality control. Our platforms support delivery of Cas nucleases and gRNAs across diverse cell types and in vivo models, ensuring high editing efficiency. With flexible production scales and expert support, we enable rapid generation of high-quality vectors for research and preclinical applications.
Important updates waiting for you!
Enter your email address below and subscribe to our newsletter