Custom Adenovirus Production Full Service

High-Titer, Research-Grade Adenovirus Production — Built to Your Specifications…

Comprehensive Adenovirus Production Services

Scalable adenovirus production solutions encompassing vector design, optional gene synthesis, adenovirus packaging, amplification, purification, and titration...

Gene Overexpression & Adenoviral Homology-Directed Repair

Gene Silencing via Adenoviral shRNA, shRNAmir, miRNA, gRNA & TuD

End-to-End Adenoviral Vector Workflow

A streamlined approach that balances speed with uncompromising scientific quality.

Ad.MAX™ Shuttle Vector Design & Cloning

Software-based adenoviral shuttle vector design for transgene or shRNA, shRNAmir, miRNA, gRNA, or TuD RNA overexpression

WEEK 1

Adenoviral Backbone Construction, Verification & QC

Gateway® transgene transfer to viral backbone followed by RE digestion & WPS of NanoPore platform

WEEK 2

Adenovirus Packaging & Multi-round Amplification

Adenoviral backbone plasmid linearization & transfection for packaging, followed by multi-round amplification

WEEK 3

Adenovirus Purification, PFU Titration & Other QCs

Adenovirus purification by gel-filtration & CsCl gradient ultracentrifugation, followed by PFU titration& other QCs

WEEK 4

Why SignaGen?

Reasons customers trust us for adenovirus production

Proven Adenovirus Expertise

10+ years of excellence | 7,000+ scientific citations | 350+ off-the-shelf adenovirus available for test

Cost-Effective & Affordable

We price-match competitors & provide an additional 10% discount

Customizable & Scalable

Final deliverables from 1000 µL to 5000 µL at >5E+10 PFU/mL for in vitro and in vivo applications

Fast Turnaround

~4-week adenovirus production full service | ~2-week adenovirus amplification service

Extensive Options of Ad.MAX™ Shuttle Vectors

Constitutively active or tissue-specific gene expression enabled by our extensive empty Ad.MAX™ shuttle vectors

Large Shuttle Vector Collection

400+ empty shuttle vectors with diverse promoters, introns, WPREs & polyA signals

Efficient Packaging of Toxic Transgenes

Our proprietary Ad.MAX system suppresses transgene expression in packaging cells during adenovirus production, enabling efficient packaging even for toxic transgenes

Custom Adenoviral Backbone Synthesis

Custom synthesis of alternative adenoviral backbones is available when off-the-shelf human type C serotype 5 (Ad5) vectors do not meet your specific requirements

All IP Belongs to Customer

Adenovirus design-related IP belongs to customer, who receives the final virus & cloned shuttle construct

Rigorous Quality Validation

qPCR Viral Particle & Genuine PFU Titer Determination

Precise quantification of viral genomes and transduction unit titrations

Purity & Identity

Silver-stained SDS-PAGE to verify adenovirus purity & transgene verification

Safety Testing

LAL assay for Endotoxins (<0.1 EU/mL), mycoplasma screening, and RCA assay

Technical Benchmarks

  • Titer: >5E+10 PFU/mL
  • Purity: Available in both in vitro and in vivo grades
  • RCA: Negative from assay via amplification in permissive cells followed by PFU titration
  • Turnaround: ~4 weeks

We Offer Fully Customizable Adenovirus Production Services

SKU #SERVICESFINAL DELIVERABLEPURITYTURNAROUND TIME
SL100736ADENOVIRUS PRODUCTION FULL SERVICE, PILOT SCALE1.0 mL at >5E+10 PFU/mLIn Vitro Grade4 weeks
SL100701ADENOVIRUS PRODUCTION FULL SERVICE, MEDIUM SCALE2.0 mL at >5E+10 PFU/mLIn Vitro Grade4 weeks
SL100702ADENOVIRUS PRODUCTION FULL SERVICE, LARGE SCALE2.0 ~ 5.0 mL at >5E+10 PFU/mLIn Vivo Grade4 weeks

Request a Quote

Discuss your project with our PhD scientists and request a quote

Contact Form SignaGen Service

We price-match competitors & provide an additional 10% discount

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