Miscellaneous - 10. page

5′-GGGCATAGATTACGTGAAA  TTCAAGAGA TTTCACGTAATCTATGCCC-3′

Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 NGG SpCas9 D1135E variant NGG (reduced NAG binding) SpCas9 VRER variant NGCG SpCas9 EQR variant NGAG SpCas9 VQR variant NGAN or […]

5′-TCC CAC GGC TTC CCC CCC GAG GTC GAG GAG CAG GCC GCC GGC ACC CTC CCC ATG AGC TGC GCC CAG GAG AGC GGC ATG GAC AGG CAC CCC […]

316 bp: gagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctat cagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaa cgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcc

Human GPR55 siRNAs: 5′-GAAUUCCGCAUGAACAUCAUU-3′ 5′-GAGAAACAGCUUUAUCGUAUU-3′ 5′-AAGAACAGGUGGCCCGAUUUU-3′ 5′-GCUACUACUUUGUCAUCAAUU-3′

iRNA sequences JAK1 #1 CCACAUAGCUGAUCUGAAA JAK1 #2 GACAUGAUAUUGAGAACGA JAK1 #3 UUACAAGGAUGACGAAGGA JAK1 #4 CGGAUGAGGUUCUAUUUCA JAK2 SP GAGCAAAGAUCCAAGACUA GCCAGAAACUUGAAACUUA GAUCCUGGCAUUAGUAUUA ACAGAAUGCUGGAACAAUA TYK2 SP GCAUCCACAUUGCACAUAA UCAAAUACCUAGCCACACU CAAUCUUGCUGACGUCUUG CACAAGGACCAACGUGUAU JAK3 SP GCGCCUAUCUUUCUCCUUU CCAGAAAUCGUAGACAUUA […]

RGS5 : CAGACUCUGCUGUUGACCUUGUCAU GGUGAACAUUGACCACUUCACUAAA RGS4: AAAGCUGCCAGUCCACAUUCAUGGU

The termination sequence for the U6 promoter is a series of T’s (5-6) in a row.

The “G” that you mentioned is in fact the “preferred” transcription start site, however, this can be replaced by any other nucleotide. It’s just that U6 prefers “G” as the […]

Background Decoy and Tough Decoy (TuD) RNAs are novel technologies for regulating RNA interference (Haraguchi et al., Nucleic Acids Res. 2009; Xie et al., Nature 2012). In short, they are […]