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Cas9 Species/Variants and PAM Sequences

Species/Variant of Cas9 PAM Sequence
Streptococcus pyogenes (SP); SpCas9 NGG
SpCas9 D1135E variant NGG (reduced NAG binding)
SpCas9 VRER variant NGCG
SpCas9 EQR variant NGAG
SpCas9 VQR variant NGAN or NGNG
Staphylococcus aureus (SA); SaCas9 NNGRRT or NNGRR(N)
Neisseria meningitidis (NM) NNNNGATT
Streptococcus thermophilus (ST) NNAGAAW
Treponema denticola (TD) NAAAAC
Additional Cas9s from various species PAM sequence may not be characterized

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