Miscellaneous - 16. page

Now we will talk about the influence of various regulations on the availability of Fetal Bovine Serum. It is a fact that import and export regulations have direct influence on […]

Dye Structure Color Vendor Cat # Price Features SYBR Green 1 Green unstable at 4 °C SYTO-82 Orange does not inhibit PCR, show no preferential binding to GC rich sequences […]

In-Fusion PCR Cloning: Clontech, http://www.freepatentsonline.com/7575860.html Cold Fusion Cloning Kit: SBI Fast Seamless Cloning Kit: Dogene CloneEZ Kit: Genescript GENEART Seamless Cloning and Assembly Kit: Invitrogen   SLIC uses the 3′ […]

1) Prepare 6 ml of 5X ISO Buffer in a 15 ml falcon tube as follows: 3 ml 1 M Tris-HCl pH 7.5 + 150µl 2 M MgCl2 + 240 µl 100 […]

Gene specific primers: GSS-F: SbfI Kozak GSS AGGCT cctgcagg CCACCATG NNNNNNNNNNNNNNNNNNNNN GSS-R: GSS * Xho1 (NNNNNNNNNNNNNNNNNNNNN TAG ctcgag ACCCAGCTTTC) For example: GAAAGCTGGGT ctcgag CTA N(15–25)   Universal Primers attL1-F ccccGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCA […]

1.Excision reaction. 44.5 mM Tris–borate (pH 8.3) 7.5 mM Tris–HCl (pH 8.0) 1.15 mM EDTA 5 mM spermidine 60 mM KCl 0.275 mg/ml BSA 15% (v/v) glycerol. Int 50~100 nM […]