atgctcgagggagtgcaggtggaGacTatctccccaggagacgggcgcaccttccccaagcgcggccagacctgcgtggtgcactacaccgggatgctt gaagatggaaagaaagttgattcctcccgggacagaaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggg gttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccact ctcgtcttcgatgtggagcttctaaaactggaatctggcggtggatccggagtcgacggatttggtgatgtcggtgctcttgagagtttgaggggaaatg cagatttggcttacatcctgagcatggagccctgtggccactgcctcattatcaacaatgtgaacttctgccgtgagtccgggctccgcacccgcactggct ccaacatcgactgtgagaagttgcggcgtcgcttctcctcgctgcatttcatggtggaggtgaagggcgacctgactgccaagaaaatggtgctggcttt gctggagctggcgcGgcaggaccacggtgctctggactgctgcgtggtggtcattctctctcacggctgtcaggccagccacctgcagttcccaggggct gtctacggcacagatggatgccctgtgtcggtcgagaagattgtgaacatcttcaatgggaccagctgccccagcctgggagggaagcccaagctcttt ttcatccaggcctgtggtggggagcagaaagaccatgggtttgaggtggcctccacttcccctgaagacgagtcccctggcagtaaccccgagccagat gccaccccgttccaggaaggtttgaggaccttcgaccagctggacgccatatctagtttgcccacacccagtgacatctttgtgtcctactctactttcccag gttttgtttcctggagggaccccaagagtggctcctggtacgttgagaccctggacgacatctttgagcagtgggctcactctgaagacctgcagtccctc ctgcttagggtcgctaatgctgtttcggtgaaagggatttataaacagatgcctggttgctttaatttcctccggaaaaaacttttctttaaaacatcagtcg actatccgtacgacgtaccagactacgcactcgactaa
Miscellaneous - 12. page
Common Epitope Tags
Common Epitope Tags Tag Protein Sequence DNA Sequence* FLAG DYKDDDDK GAC TAC AAA GAC GAT GAC GAC AAG HA YPYDVPDYA TAC CCA TAC GAT GTT CCA GAT TAC GCT His […]
pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis.
atggtgagcaagggcgaggagaataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggcca cgagttcgagatcgagggcgagggcgagggccgcccctacgaggcctttcagaccgctaagctgaaggtgaccaagggtggccccctgcc cttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggtctacattaagcacccagccgacatccccgactacttcaagctgtcct tccccgagggcttcaggtgggagcgcgtgatgaacttcgaggacggcggcattattcacgttaaccaggactcctccctgcaggacggcgt gttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctccgag gagcggatgtaccccgaggacggcgccctgaagagcgagattaagtataggctgaagctgaaggacggcggccactacgccgccgaggt caagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacatcgtggacatcaagttggacatcgtgtcccacaacgaggacta caccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagGctagCTAA
mEos3.2
Monomeric variant of green-to-red photoswitchable fluorescent protein EosFP, engineered for reduced aggregation relative to mEos2. ATGAGTGCGATTAAGCCAGACATGAAGATCAAACTCCGTATGGAAGGCAACGTAAACGGGCACCACTTTGTGATCGACGGAGATGGTACAGGCAAGCC TTTTGAGGGAAAACAGAGTATGGATCTTGAAGTCAAAGAGGGCGGACCTCTGCCTTTTGCCTTTGATATCCTGACCACTGCATTCCATTACGGCAACAGG GTATTCGCCAAATATCCAGACAACATACAAGACTATTTTAAGCAGTCGTTTCCTAAGGGGTATTCGTGGGAACGAAGCTTGACTTTCGAAGACGGGGGC ATTTGCAACGCCAGAAACGACATAACAATGGAAGGGGACACTTTCTATAATAAAGTTCGATTTTATGGTACCAACTTTCCCGCCAATGGTCCAGTTATGC AGAAGAAGACGCTGAAATGGGAGCCCTCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGACGGGTGATATTGAGATGGCTTTGTTGCTTGAAGGA AATGCCCATTACCGATGTGACTTCAGAACTACTTACAAAGCTAAGGAGAAGGGTGTCAAGTTACCAGGCGCCCACTTTGTGGACCACTGCATTGAGATT TTAAGCCATGACAAAGATTACAACAAGGTTAAGCTGTATGAGCATGCTGTTGCTCATTCTGGATTGCCTGACAATGCCAGACGATAA
Transfection Techniques for Neuronal Cells
Summary of advantages and disadvantages of different techniques commonly used to transfect mammalian neurons Best suited for Strengths Limitations Toxicity Onset, level, and duration of expressiona Maximum insert size Genome […]
mIFP Full Sequence
atgtcggtaccgctgactacctcagcattcggccacgcgtttctggctaactgtgaacgcgagcagatccacctggcgggctccattcagccgcac ggtatcctgctggctgtgaaagagccggacaacgtggtgatccaggcttctattaacgctgcggagttcctgaacaccaactctgttgttggcc gtccgctgcgtgacctgggcggcgatctgcctttgcagatcctgccgcacctgaacggcccgctgcacctggctccgatgaccctgcgttgtacc gtgggttctccgccgcgtcgtgtggactgtaccattcatcgtccgtctaacggcggcctgatcgtagaactggaaccagcaaccaagaccact aacattgcgccggctctggacggtgcgtttcatcgtatcacttcttcatcctccctgatgggcctgtgtgacgaaaccgcgactattatccgtgaga ttactggctacgaccgtgtgatggtagtacgtttcgatgaagagggtaatggcgaaattctgtccgaacgtcgtcgtgcggacctggaagcgtt cctgggtaaccgctacccggcgtctactattccgcagatcgctcgtcgcctgtacgaacataaccgtgttcgcctgctggtagatgtgaactat actccggttccgctacagccgcgcatcagcccgctgaacggtcgtgatctggatatgtccctgtcttgcctgcgctctatgtccccgatccacca gaaatacatgcaggacatgggcgttggcgcgaccctggtttgctctctgatggtgtctggtcgtctgtggggtctgatcgcttgccaccacta cgaaccgcgcttcgttccgttccacattcgcgctgctggcgaagcgctggcggaaacttgtgcgatccgcatcgcgacgctggagagcttt gcacagtctcagtccaaatga
Infrared fluorescent proteins
Hunting for new fluorescent proteins in the coral reefs of the Caribbean and Australia is a task that a lucky few researchers have managed to get funding for. Scuba diving […]
NLSs
SV 40 Nucleoplasmin: AVKRPAATKKAGQAKKKKLD EGL-13: MSRRRKANPTKLSENAKKLAKEVEN c-Myc: PAAKRVKLD TUS-protein: KLKIKRPVK
Make a protein expression predominantly in membrane
A Kozak consensus sequence (CCAACCATGG) and Src myristoylation sequence (MGSSKSKPKDPSQR) are added to the N-terminal of ORF of specific gene.