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Cell Culture Antibiotic Selection Guide

Product NameGram (+)bacteriaGram (-)bacteriaYeastsMoldsMycoplasmaSuggested Working Conc.
Amphotericin B   2.5 mg/L
Amphotericin B-Solubilized (Approx. 45%)   5.6 mg/L (of solid)
Amphotericin B(250 μg/ml solution)   10 ml/L
Ampicillin   100 mg/L
Antibiotic Antimycotic Solution (100x)(10,000 units penicillin, 10 mg streptomycin, and 25 µg amphotericin B per ml) 10 ml/L
Cephalothin   100 mg/L
Dihydrostreptomycin   100 mg/L
Erythromycin   100 mg/L
Gentamicin Sulfate  50 mg/L
Gentamicin Sulfate (10 mg/ml solution)  5 ml/L
Gentamicin Sulfate (50 mg/ml solution)  1 ml/L
L-Glutamine-Penicillin-Streptomycin Solution (200 mM L- glutamine, 10,000 units penicillin, and 10 mg streptomycinper ml)   10 ml/L
Kanamycin Monosulfate  100 ml/L
Kanamycin Sulfate (10 mg/ml solution)  10 ml/L
Kanamycin Sulfate (50 mg/ml solution)  2 ml/L
Lincomycin HCI    100 mg/L
Neomycin Sulfate   50 mg/L
Neomycin Sulfate (10 mg/ml solution)   5 ml/L
Nystatin (5,000 units nystatin per mg)   2.5 X 105U/L (50 mg/L)
Nystatin   50 mg/L
Nystatin Suspension   24 ml/L
Paromomycin Sulfate   100mg mg/L
Penicillin-G (potassium salt)    100,00 U/L
Penicillin-G (sodium salt)    100,000 U/L
Penicillin-Streptomycin solution (5,000 units penicillin-G and 5 mg streptomycin per ml)   20 ml/L
Penicillin-Streptomycin Solution (10,000 units penicillin-G and 10 mg streptomycin per ml)   10 mg/L
Penicillin-Streptomycin-Neomycin solution (5,000 units penicillin-G,5 mg streptomycin and 10 mg neomycin per ml)   10 ml/L
Phenoxymethylpenicillinic Acid (potassium salt) [Penicillin V]    100,000 U/L
Polymyxin B Sulfate    50 mg/L
SpectinomycinDihydrochloride   7.5-20 mg/L
Spectromycin Sulfate   10 mg/L
TetracyclineHydrochloride   10 mg/L
Tylosin Tartrate(8 mg/ml soln.)   1 ml/L
ANTIBIOTICS FOR PLANT CELL CULTURE
Product NameGram (+)bacteriaGram (-)bacteriaMycobacteriaYeastsMoldsMycoplasma
Carbenicillin    
Cefotaxime    
Chloramphenicol  
G 418      
Gentamicin Sulfate   
Hygromycin B      
Kanamycin   
Paromycin    
Penicillin G, Potassium    
Penicillin G, Sodium    
Rifampicin   
Streptomycin Sulfate   
Vancomycin     
Product NameGram (+)bacteriaGram (-)bacteriaMyco-bacteriaYeastsMoldsMyco-plasma
Nystatin    
Pentachloronitrobenzene     
Thiabendazole     
† = Effective against most species
‡ = Effective against certain species
1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant
selection; SE = somatic embryogenesis; SSC = stem selection culture; PC = protoplast culture; TEC = tuber explant culture;
STM = shoot tip micropropagation
ANTIBIOTIC SELECTION AGENTS FOR CELL CULTURE
Product NameMode of ActionSuggested Working Conc.
Actinomycin DComplexes with DNA and interferes with RNA synthesis1 µg/ml
Bleomycin SulfateComplexes with DNA, causing strand scissions10-100 µg/ml
ChloramphenicolInhibits elongation at peptidyl transferase5 µg/ml
G 418Blocks polypeptide synthesis and inhibits chain elongation100-800 µg/ml
G 418 (50 mg/ml solution)Blocks polypeptide synthesis and inhibits chain elongation1 µg/ml
Hygromycin BBlocks polypeptide synthesis and inhibits chain elongation 
Mitomycin CInhibits nucleic acid synthesis10-50 µg/ml
Mycophenolic AcidBlocks inosine monophosphate dehydrogenase in guanosine monophosphate pathway25 µg/ml
Puromycin HClInhibits protein synthesis10-100 µg/ml

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