Skip to content

Three Validated siRNAs

COX-2 siRNA: 5-AACTGCTCAACACCGGAATTTTT-3 COX-1 siRNA: 5-AAGTGCCATCCAAACTCTATCTT-3 15-PGDH siRNA: 5-AACAGTGGTGTGAGACTGAAT-3

Validated siRNA Targeting 15-PGDH and HIF-alpha

15-PGDH and Scramble control siRNA sequence: 15-PGDH: (NM_001034419.2) 5′-GCUUUGAA UGGUGCUAUUATT-3′ Scramble: 5′-UUCUCCGAACGUGUCAC GUTT-3′ HIF-1α siRNA: (NM_174339.3) 5′-GCUCCUGAGGAAGAACUAATT-3′ Scramble: 5′-UUCUCCG AACGUGUCACGUTT-3′

Validated Human FOXO1 siRNA

Human FOXO1 siRNAs: 5′-GGAGGUAUGAGUCAGUAUATT-3′ (sense) 5 ′-UAUACUGACUCAUACCUCCTT-3 ′ (antisense),  

Two Validated Gadd45a siRNAs

Two validated Gadd45a siRNAs siRNA1: sense: 5′- GAG CAG AAG ACC GAA AGG AUG GAU A-3′; anti-sense: 5′-UAU CCA UCC UUU CGG UCU UCU GCU C-3′ siRNA2: sense 5′-GGU GAC […]

Primers for rAAV qPCR Titration

Cytomegalovirus (CMV) Promoter Assay Forward: 5-CATCTACGTATTAGTCATCGCTATTACCA-3 Reverse: 5-GAAATCCCCGTGAGTCAAACC-3 Probe: 5-6FAM-TCAATGGGCGTGGATAG-MGBNFQ-3 Human Thyroxine-binding Globulin (TBG) Promoter Assay Forward: 5-AAACTGCCAATTCCACTGCTG-3 Reverse: 5-CCATAGGCAAAAGCACCAAGA-3 Probe: 5-6FAM-TTGGCCCAATAGTGAGAACTTTTTCCTGC-TAMRA-3 Enhanced Green Fluorescent Protein (eGFP) Assay Forward: 5-AGCAAAGACCCCAACGAGAA-3 […]

rAAV Average Molecular Weight

Adeno-associated virus (AAV) is a parvovirus and is composed of three structural proteins and a linear, single-stranded DNA (ssDNA) genome of approximately 4.7 kb (17). The particle has icosahedral symmetry and a […]