15-PGDH and Scramble control siRNA sequence: 15-PGDH: (NM_001034419.2) 5′-GCUUUGAA UGGUGCUAUUATT-3′ Scramble: 5′-UUCUCCGAACGUGUCAC GUTT-3′ HIF-1α siRNA: (NM_174339.3) 5′-GCUCCUGAGGAAGAACUAATT-3′ Scramble: 5′-UUCUCCG AACGUGUCACGUTT-3′
Miscellaneous - 9. page
15-PGDH-specific shRNAs Target Region Sequence
15PGDH-specific shRNAs target region sequence: 1) CACAGCCATCCTTGAATCAAT 2) GAAGGCGGCATCATTATCAAT 3) GCAACAACTGAGAGACACTTT 4) CTGGAGTGAATAATGAGAAA 5) ACTCATAACAACACAGACATA
Validated Mouse FOXO3 siRNA
Mouse FOXO3 siRNA: 5′-CCAGUGACUUGGACCUGGACAUGUU-3′
Validated Mouse FOXO1 siRNA
Mouse FOXO1 siRNA 5′-GCGGGCUGGAAGAAUUCAATT-3′
Validated Human FOXO1 siRNA
Human FOXO1 siRNAs: 5′-GGAGGUAUGAGUCAGUAUATT-3′ (sense) 5 ′-UAUACUGACUCAUACCUCCTT-3 ′ (antisense),
Human Period 2 (hPer2) Target Sequences
Human Period 2 (hPer2) target sequences were as follows: i) 5’-CAACAAGGTGCTGAGAGTCAGCTTT-3’ ii) 5’-ACTCTGGTTATGAAGCCCCTAGAAT-3’
Two Validated Gadd45a siRNAs
Two validated Gadd45a siRNAs siRNA1: sense: 5′- GAG CAG AAG ACC GAA AGG AUG GAU A-3′; anti-sense: 5′-UAU CCA UCC UUU CGG UCU UCU GCU C-3′ siRNA2: sense 5′-GGU GAC […]
CK0.4 Promoter
ACGCGTAACTTGTGGACTAAGTTTGTTCACATCCCCTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCA CACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAGGTGTTGGGGAGGCAGTTACCGGG GCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGCGAGGCTGTGAGCAGCCACAGTGCCCTGCTC AGAAGCCCCAAGCTCGTCAGTCAAGCCGGTTCTCCGTTTGCACTCAGGAGCACGGGCAGGCGAGTGGCCCCTAGTTCTGGGGGCAGCTCTAGAG CCGATCCCCGGGTACCGGT
Secreted Gaussia Luciferase ORF
ATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCCAAGCCCACCGAGAACAACGAAGACTTCAACATCGTGGCCGTGGCCAG CAACTTCGCGACCACGGATCTCGATGCTGACCGCGGGAAGTTGCCCGGCAAGAAGCTGCCGCTGGAGGTGCTCAAAGAGATGGAAGCCAATGCCCGG AAAGCTGGCTGCACCAGGGGCTGTCTGATCTGCCTGTCCCACATCAAGTGCACGCCCAAGATGAAGAAGTTCATCCCAGGACGCTGCCACACCTACGA AGGCGACAAAGAGTCCGCACAGGGCGGCATAGGCGAGGCGATCGTCGACATTCCTGAGATTCCTGGGTTCAAGGACTTGGAGCCCATGGAGCAGTTCA TCGCACAGGTCGATCTGTGTGTGGACTGCACAACTGGCTGCCTCAAAGGGCTTGCCAACGTGCAGTGTTCTGACCTGCTCAAGAAGTGGCTGCCGCAAC GCTGTGCGACCTTTGCCAGCAAGATCCAGGGCCAGGTGGACAAGATCAAGGGGGCCGGTGGTGACTAA
NCOR1 and MSTN Validated Target Region Sequence
NCOR1 target: GCTCTCAAAGTTCAGACTCTT MSTN target: GATTGGATTATCGCTCCTAAA