Small interfering RNA (siRNA),
sometimes known as short interfering RNA or silencing RNA, has a well-defined
structure: a short (usually 21-nt) double strand of RNA (dsRNA) with 2-nt 3'
overhangs on either end. siRNAs can be introduced into cells by various
transfection methods to bring about the specific knockdown of a gene of
interest. Essentially any gene of which the sequence is known can thus be
targeted based on sequence complementarity with an appropriately tailored siRNA.
This has made siRNAs an important and indispensable tool for gene function and
drug target validation studies in the post-genomic era.
While liposome
or polymer reagents often give very good DNA delivery efficacy, they often failed to
efficiently drive siRNA into mammalian cells due to the length of
the siRNA anionic segment that is too short to maintain electrostatic cohesion
with the cationic lipids or polycationic polymer. We
modified the liposome and polymer by addition of specific pre-screened
hydrophobic groups which confers the pH dependent conformational changes (PDCC)
at physiological pH condition and greatly stabilizes siRNA lipolplex or polyplex.
Based
on our unique and proprietary “PDCC” technology, currently we are developing and
manufacturing four categories of siRNA transfection reagents, which show
distinct transfection characteristics with the leading products in the market.
Guideline to choose a a siRNA transfection reagent for your purpose:
|
Reagents |
Chemistry |
Features |
|
- PepMute™ |
Simulated CCP |
95% Knockdown at as lower as 0.5 pmol (1.0 nM) on commonly used cells, best for siRNA transfection |
|
- GenMute™ |
PDCC modified polymer |
94% Knockdown at as lower as 0.5 pmol (1.0 nM) on commonly used cells, best for siRNA single transfection or siRNA/DNA co-transfection |
|
- GenJet™ |
PDCC modified liposome and polymer hybrid |
95% Knockdown at as lower as 0.5 pmol (1.0 nM) on commonly used cells and hard-to-transfect cells |
|
PDCC modified liposome |
95% Knockdown at as lower as 0.5 pmol (1.0 nM) on commonly used cells and hard-to-transfect cells |
|
|
PDCC modified CCP |
95% Knockdown at as lower as 0.5 pmol (1.0 nM) on commonly used cells and hard-to-transfect cells, best for siRNA single transfection and siRNA/DNA co-transfection to hard-to-transfect cells |
|
