PepMute™ Plus siRNA Transfection Reagent
Description:
PepMute™
Plus siRNA & DNA Transfection Reagent is an upgraded version of PepMute™
reagent (Cat # SL100566) by crosslinking pre-screened hydrophobic amino acids to
its backbone. With
our proprietary pH Dependent Conformational Change
(PDCC) technology, the transfection reagent
which contains 36 amino acids long's peptide backbone was formulated by addition of pre-screened hydrophobic
amino acids to all amine groups, conferring
PepMute™
Plus reagent an ability to self-assembly during formation of transfection
complex and
making PepMute™ reagent
a versatile and most powerful gene delivery tool which
provides more than 95% silencing efficiency at 1 nM siRNA in
variety of mammalian cells. PepMute™
Plus Reagent have been validated to effectively and
reproducibly transfect single DNA or siRNA or co-transfect DNA/siRNA to variety
of mammalian cells.
Figure 1. A cartoon showing
PepMute™
Plus siRNA Transfection Reagent was developed
Size & content:
-
PepMute™ Plus Transfection Reagent,
1.0 mL, sufficient for ~1000 reactions based on transfecting 2.5 pmols siRNA in
24-well plate
-
PepMute™ Plus Transfection Buffer(5x ), formulated for maximal transfection
efficiency, 8.0 mL
Storage:
Store at 4 °C for PepMute™
Plus
Reagent and RT for PepMute™
Transfection Buffer (5x ). If stored properly, the product is stable for 12 months or
longer.
Advantages:
- Effective knockdown (95%) with only 0.5 pmol siRNA (1.0 nM siRNA in 24-well plate)
- Formulated for hard-to-transfect mammalian cells
- One tube reaction and easy transfection protocol
- Best for broad spectrum especially
hard-to-transfect mammalian cells
- Very low cytotoxicity
Comparisons of Knockdown Efficacy of PepMute™ Plus siRNA & DNA Transfection Reagent with
Brand Name Products
Figure 2. Knockdown efficacy
comparison of PepMute™ Plus Transfection Reagent (upper panel) vs. Dharmafect™ 4
siRNA Transfection Reagent (middle panel)
and Lipofectamine™
2000 (lower panel) on HEK293 cells. siRNA targeting renilla luciferase
at different final concentrations ranging from 0.5 to 20 nM was co-transfected with renilla luciferase
gene (0.5 µg of pRL-CMV DNA per well) by the above three transfection reagents
per manufacturers' protocols into HEK293 cells growing on a 24-well plate.
Renilla luciferase activity was determined 24h after
post co-transfection with renilla luciferase determination system (Promega). The
luminescence was measured from 5.0 µl of lysate during 10s integration with a
luminometer (Beckman Coulter LD 400). Luciferase activity was
expressed as light units integrated over 10s (RLU) and normalized per mg of cell
protein by using the BCA assay. The errors bars represent standard deviation
derived from triplicate experiments. Luciferase-silencing efficiency was
calculated relative to untreated cells. While
PepMute™ Plus and Dharmafect™ 4 reagents delivered
significant gene silencing from 1.0 nM of renilla luciferase siRNA, lipofectamine™
2000 gave good knockdown only after 30 nM (data not shown).
Figure 3. PepMute™ Plus Transfection Reagent knocked down GFP gene expression in HepG2 cells.
GFP cDNA, pEGFP-N3, was co-transfect with a siRNA targeting
GFP gene (final 5.0 nM, right panel) and a sham siRNA (final 5.0 nM, left panel)
into HepG2 cells by
PepMute™ Plus Transfection Reagent. GFP
fluorescence was visualized 24h post transfection with a Nikon T2000
fluorescence micorscopy. Quantitative analysis showed that
GFP siRNA at 5.0 nM delivered
by PepMute™ Plus Transfection Reagent knocked down 96% co-transfected GFP expression in HepG2 cells.
Data Sheet & Protocol
-
A Protocol for DNA/siRNA Co-Tranafection to Mammalian
Cells
-
A Protocol for siRNA Transfection to Mammalian Cells
-
A Protocol for DNA Transfection to Mammalian Cells
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enter your shipping address and email us at
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