shRNA/miRNA/gRNA AAV Production Service:
Adeno-associated virus (AAV) is a small virus (20 nm in diameters) replication-defective, nonenveloped virus, which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. AAV can infect both dividing and non-dividing cells and may incorporate its genome into that of the host cell.
With our proprietary genetically engineered AAV·HT™ packaging cell, we are offering custom shRNA/miRNA/gRNA rAAV vector production services (conventional single stranded or double-stranded also known as self-complementary) listed below to fit your different research needs.
|
Catalog # |
Service Items |
Final Deliverable * |
rAAV Purity |
Lead Time |
|
SL100810 |
1.0 mL at >1E+13 VG/mL |
In Vivo Grade |
3~4 weeks |
|
|
SL100871 |
shRNA/miRNA/gRNA rAAV Production, Full Service, Medium Scale |
0.2 mL at >1E+13 VG/mL |
In Vivo Grade |
3~4 weeks |
|
SL100874 |
0.1 mL at >1E+13 VG/mL |
In Vivo Grade |
3~4 weeks |
|
|
SL100872 |
1.0 mL at >1E+13 VG/mL |
In Vivo Grade |
1~2 weeks |
|
|
SL100873 |
0.2 mL at >1E+13 VG/mL |
In Vivo Grade |
1~2 weeks |
|
|
SL100875 |
>6xshRNA rAAV cis Plasmids with >1 Plasmid at 80% KD |
N/A |
2~3 weeks |
Large shRNA/miRNA/gRNA AAV cis vector inventory for constructing AAV cis plasmid with a specific promoter and reporter:
| For Conventional shRNA/miRNA/TuD rAAV Production |
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|
Single-promoter shRNA/miRNA AAV cis vector |
Dual-promoter shRNA/miRNA rAAV cis vector |
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|
Promoterless |
|
Promoterless |
CMV |
eGFP |
|
| For CRISPR/Cas9 rAAV Production |
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|
Single-promoter CRISPR/Cas9 AAV cis vector |
Dual-promoter CRISPR/Cas9 AAV cis vector |
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|
Promoterless |
Empty |
Cas9 hCas9 SpCas9 hSpCas9 SaCas9 AsCpf1 hAsCpf1 ftCas9 st1Cas9 cjCas9 cdCas9 ciCas9 ncCas9 st3Cas9 nmCas9 mgCas9 nsCas9 |
Promoterless |
CMV |
Cas9 |
Required Materials: GenBank access #, target sequence, or validated siRNA, shRNA sequence; miRNA accession # for TuD, pre-miRNA or mature miRNA or gRNA sequence.
Deliverables: Total >1.0 ml at >1013 VG/mL*** shRNA/miRNA/gRNA rAAV for large scale production.
With our proprietary genetically engineered AAV·HT™ packaging cell and a modified rAAV cis plasmid, the shRNA/miRNA rAAV yield is easy to reach super high level--------total 1E+15 VG.
For super high level rAAV production up to 1E+15 VG, please contact us to request a quote.
* Some terms may apply for custom double-stranded or self-complementary rAAV production service.
** If commercial shRNAs or siRNAs, gRNAs of your target gene or tough decoy (TuD) miRNA are not available or non-functional, or your project requires special design for further development, we offer special shRNA/gRNA/TuD design without additional charge!
*** Final viral yield may depend on the transgene in the virus.
