Description
GenJet™ DNA In Vitro
Transfection Reagent for Rin is pre-optimized znd conditioned for transfecting
Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on Rin related cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
Transfection Medium
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~90%
DMEM with 4.5 g/L glucose, 10% FBS
DMEM with 4.5 g/L glucose, 1% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes in presence of 1% FBS
No
48 hours
EGFP
pEGFP-N3 (CMV promoter)
~85% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on Rin-5F Cells.
Rin-5F cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
