Description
GenJet™ DNA In Vitro
Transfection Reagent for RAW 264.7 Cell is pre-optimized for transfecting
RAW 264.7 cells. RAW 264.7 cells are a macrophage-like, Abelson leukemia
virus transformed cell line derived from BALB/c mice.
RAW 264.7 cell slide is often used for immunocytochemical analysis of protein
and protein-protein interaction. Procedures for immunohistochemistry can
be directly applied. GenJet™ reagent, 1.0 ml, is
sufficient for 300 to 600 transfections in 24 well plates or 50 to 100
transfections in 6 well plates.
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Summary
of Optimal Transfection Conditions:
Confluence on
the day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~80%
DMEM with 10% FBS
3:1
1xGenJet™ Transfection Buffer
10 minutes at RT
Yes
No
48 hours
EGFP
pEGFP-N3 (CMV promoter)
75% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet & Protocol
