Contents Per Kit:
●
1x1.0 ml of GenJet™
DNA Transfection Reagent
● 1x8.0 ml (x5 ) of GenJetll™ Transfection Buffer
Description
GenJet™ DNA In Vitro
Transfection Reagent for HUVEC is pre-optimized for
transfecting human umbilical vein endothelial cells
(HUVEC). HUVEC is one of
hard-to-transfect cell lines partly due to the transfection toxicity related
cell death. In combination
of GenJet™ Transfection Buffer, the pre-optimized transfection
reagent gave up to 60% efficiency.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on HUVEC. GenJet™ reagent, 1.0 ml, is sufficient for 500 transfections in 24 well plates or
250 transfections in 6 well plates.
|
Summary of
Optimal Shaved CellTransfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of shaving
Cell density for plating
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
DMEM with 4.5 g/L glucose, 10% FBS
70%
1.3x10^5 cells per square centimeter
2.25:1
GenJet™
Transfection Buffer (1x )
10 minutes at RT
48 hours
EGFP
pEGFP-N3 (CMV promoter)
56% |
Storage Condition
Store at 4 0C. If stored properly, the product is stable for 12 months or longer
Data Sheet
