GenJet™ In Vitro DNA Transfection Reagent
Description
GenJet™ DNA In Vitro
Tranfection Reagent is a powerful transfection reagent that ensures effective
and reproducible transfection with invisible toxicity. GenJet™ is formulated
by unique chemistry (covalently cross-linking cationic lipids with
polymer), giving rise to exceptional transfection efficiency
with distinguishable features in comparison of other types of reagents. GenJet™ was shown to deliver genes to various
established cell lines as well as primary cells including HEK293, 293T, 293E,
CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of
other eucaryotic cell lines. GenJet™ reagent, 1.0 ml, is sufficient for ~666 transfections in 24 well plates or
~333 transfections in 6 well plates.
Application
- Deliver DNA/RNA into
mammalian, insect cells
- Greatly enhance (up to 200 times higher efficiency)
virus (e.g., replication-deficient Adenovirus) infection
of hard-to-transfect cells
Features
- Exceptional high titers of
virus production
- Equally good for very long DNAs
(up to 56 kb)
- Equally good for single or
multiple DNA transfections
- Exceptional high levels of recombinant protein production
- Top choice for hard-to-transfect cells
- Inexpensive transfection reagent
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Broad Transfection Spectrum for
Mammalian Cell Types
Cell
Lines |
Transfection Efficiency
(% GFP) |
Cell
Lines |
Transfection Efficiency
(% GFP) |
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo-2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
McArdle 7777
Hep3D
SHEP
MDA-MB-231
3T3-442A
COS-7
CV-1
D 407
DHD Pro.b
LS180 |
51%
72%
52%
86%
78%
32%
83%
42%
80-90%
81%
50-65%
49%
22%
38%
20%
60%
78%
71%
38%
10%
60-70%
53%
62%
51%
46% |
K562
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
SH-SY5Y
SiHa
SKOV3
HUVEC
IGROV1
Jurkat
6CSFMEo
WEHI 231
SAOS-2
SN56
MC3T3-E1
BT474
C2C12
Primary melanocyte
Hela-S3
A549
Hep G2 |
22%
52%
51%
52%
72%
88%
34%
14%
62%
52%
51%
21%
5%-15%
73%
25%
52%
69%
70%
46%
46%
46%
78%
78%
58% |
Comparisons of Transfection Efficiency and Cytotoxicity of GenJet™ DNA In Vitro
Transfection Reagent with the Leading Brand Name Products
Exceptional transfection efficiency of extremely
long DNA by GenJet™ DNA In Vitro Transfection Reagent. HEK293 cells were transfected with GFP tagged DNA (total 14 kb) by GenJet™ (upper panel) and
FuGene™ 6 (lower panel) respectively. The
cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase
(left panel) and FITC (right panel) imaging 24 hours post transfection
Exceptional high titers of lentivirus generated
from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left
panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter
virus supernatant from 293F cells were used to counter-infected 293F cells which
was then passed FACS. The numbers at the upper right corner indicate the
percentage of transduced cells. The titers of lentivirus generated with GenJet™
and L2K were quantified to be 7.9x106 and 3.1x106 tu/ml
respectively
Primary Murine Skin
Fibroblast transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA
Transfection Reagent (upper panel) and the most popular brand product FuGene™ HD
(lower panel). The cells were visualized by Nikon Eclipse Fluorescence
microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24
hours post-transfection
Primary Murine
Keratinocytes transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA
Transfection Reagent (upper panel) and one of the most popular brand product
FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse
Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging
(right panel) 24 hours post-transfection
Comparison of cytotoxicity of GenJet™ DNA In Vitro
Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary
murine fibroblast was incubated with the indicated transfection
reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High
Glucose medium followed by replacement of complete serum-containing medium. The
cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase
imaging 24 hours post transfection
Technical Information &
Datasheet
- GenJet™ In
Vitro DNA Transfection Datasheet
- GenJet™ MSDS