Description:
GenMute™
siRNA Transfection Reagent for Primary Neurons is pre-optimized for transfecting
siRNA to primary neurons. Primary neurons are notoriously heard to
transfect. With the pre-optimized
siRNA transfection conditions, GenMute™ reagent was
confirmed to give up to 90% silencing at final 50 nM siRNA on primary cultured
neurons.
Size & content:
-
GenMute™ Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 25 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on
primary neurons. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of transfection
Optimal siRNA concentration
Optima transfection timing
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA
Complex
Maximal Silencing Efficiency
Transfection
Results:
Gene silenced
Silencing
Efficiency (% ) |
Nuerobasal plus B27 serum free medium
~95%
25 nM final
5 ~ 6 days culture
1xGenMute™
Transfection Buffer
~15 minutes at RT
~48 hours
Beclin-1
80% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
