Custom Adenovirus Production Full Service, Large Scale
Production of Recombinant Serotype 5 Adenovirus (E1/E3 deletion) with Ad.MAX System:
- Unsurpassable transduction efficiency (nearly 100% vs. AAV 30~40% and Lentivirus ~30%) in most mammalian cells.
- No integration into host genome.
- Compatible with toxic gene of interest (GOI).
- Induce high-level transient protein expression.
- Accommodate inserts of up to 7.5 kb.
Large collection of Ad.MAX™ shuttle vector for cloning shuttle plasmid with a specific promoter and reporter:
|
Single promoter Ad.MAX™ shuttle vector
|
Dual promoter Ad.MAX™ shuttle vector 
|
|
Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
ALB(1.4)
ApoE/AAT1
CaMKII
ELA1
Enh358MCK
cTNT
GFAP
MBP
SST
TBG
αMHC
hRPE
mIP1
tMCK
|
|
Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
ALB(1.4)
ApoE/AAT1
CaMKII
ELA1
Enh358MCK
cTNT
GFAP
MBP
SST
TBG
αMHC
hRPE
mIP1
tMCK
|
Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
CaMKII
cTNT
GFAP
|
eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ
|
Service Description:
1. Clone your gene of interest (GOI) into a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Initial adenoviral stock production.
3. Adenovirus amplification from ~4000 cm2 Ad.MAX™ 293 cells.
4. Adenovirus purification via 2xCsCl ultracentrifugation.
5. Recombinant adenovirus PFU titration.
Required Materials: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).
Turnaround Time: 3 ~ 4 weeks.
Deliverables: >2.0 mL (10x0.2 mL) super purified in vivo grade of adenoviral particles at >5E+10~5E+11 PFU/mL*.
We offer discount for new customers, please request a quote with us today.
We offer truncated service as well, please request a quote with us today.
* Final adenovirus yield may depend on the nature of transgene.
