Description
GenJet™ DNA In Vitro
Transfection Reagent for Hela is pre-optimized for Hela cell transfection.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to
1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes
48 hours
EGFP
pEGFP-N3 (CMV promoter)
88% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on Hela Cells
GenJet™ reagent is optimized for Hela
cell (ATCC # CCL-2.2). Hela cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 48 hours post transfection
Data Sheet
