Description
GenJet™ DNA In Vitro
Transfection Reagent for keratinocytes is pre-optimized for transfecting
human or mouse primary or immortalized keratinocytes.
Keratinocytes are hard to transfect partly due to the transfection toxicity
related cell death. The pre-optimized transfection
reagent gave up to 50 % efficiency with minimal cell death.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on keratinocytes. GenJet™ reagent, 1.0 ml, is sufficient for
~600 transfections in 24 well plates or ~300 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~18 Hours After Transfection
48 hours
EGFP
pEGFP-N3 (CMV promoter)
56% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
