1. Extract RNA using a commercial kit
2. DNase treatment using RNase free DNase 1U/ul from Promega to digest residual plasmid DNA
3. Use ABI TaqMan One-Step RT-PCR master mix for RT-PCR.

RT: (30 minutes at 48°C) using MultiscribeT Reverse Transcriptase (MuLV)
PCR: 40 cycles at 95°C for 15 seconds, followed by 1 minute at 60°C.
Use one ‘no amplification control’ (NAC) for every sample (sample without RT-enzyme)

Primers:
eGFP: 5’- GGAGCGCACGATCTTCTTCA-3’ + 5’-AGGGTGTCGCCCTCGAA-3’
LTR:  5′-TGTGTGCCCGTCTGTTGTGT-3′ and 5′-GAGTCCTGCGTCGAGAGAGC-3′
WPRE: 5′-CCGTTGTCAGGCAACGTG-3′ and 5′-AGCTGACAGGTGGTGGCAAT-3′

TaqMan probe:
eGFP: 5’-FAM-CTACAAGACCCGCGCCGAGGTG-TAMRA-3’
LTR: 5′-FAM-CAGTGGCGCCCGAACAGGGA-TAMRA-3′
WPRE: 5′-FAMTGCTGACGCAACCCCCACTGGT-TAMRA-3′