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Daily Archives: October 13, 2015

Role of Serum in Cell Culture Media

Serum provides > growth factors and hormones > binding and transport proteins > attachment and spreading factors > additional amino acids, vitamins and trace elements > fatty acids and lipids > […]

Lentivirus Production Protocol

Supplies: 293FT Cells (Invitrogen: R700-07) T-225 tissue culture flasks (Nunc: 159934) T-75 tissue culture flasks (Nunc: 156499) 500 cm2 tissue culture plates (Nunc: 166508) Ultracentrifuge tubes (Beckman Culture: 344058) DMEM […]

Plasmids for Optogenetics Sensors from Addgene

Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics […]

Optogenetics rAAVs

INTRSECT (INTronic Recombinase Sites Enabling Combinatorial Targeting)-related constructs A versatile single-AAV system for selective expression that is conditional upon multiple cell-type features (such as wiring or genetic type) related by […]

Fetal Bovine Serum – the Tipping Point

Users of US Fetal Bovine Serum have been watching for months and months how prices climb up along with a constantly growing shortage and with no end in sight. Yet […]