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Day October 9, 2015
Real Time PCR Dyes
Dye Structure Color Vendor Cat # Price Features SYBR Green 1 Green unstable at…
Primers for Lentivirus Titration using qRT-PCR
Vector and region amplified Primers and probes dedicated for the amplification of lentiviral or…
Fusion Cloning, Phusion Cloning, Pfusion Cloning and Confusion PCR Cloning
In-Fusion PCR Cloning: Clontech, Cold Fusion Cloning Kit: SBI Fast Seamless Cloning Kit:…
Gibson Assembly Mix for joining two or more PCR fragments
1) Prepare 6 ml of 5X ISO Buffer in a 15 ml falcon tube as follows:…
Rapid one-step LR recombinational cloning using an enzyme mixture containing Int, IHF and Xis (Gateway Clonase) and two step PCR amplification
Gene specific primers: GSS-F: SbfI Kozak GSS AGGCT cctgcagg CCACCATG NNNNNNNNNNNNNNNNNNNNN GSS-R: GSS *…
Home-made Gateway Clonase II
1.Excision reaction. 44.5 mM Tris–borate (pH 8.3) 7.5 mM Tris–HCl (pH 8.0) 1.15 mM…
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