Product Category: human Adenovirus
Type5 (dE1/E3), mCherry-PA-Rac1 adenovirus for over-expression of
mCherry-PA-Rac1 under CMV promoter; Ad-CMV-mCherry-PA-Rac1
Description: Rac1 is a key GTPase regulating actin cytoskeletal dynamics in
metazoan cells. Rac1 mutants were fused to the photoreactive LOV (light oxygen
voltage) domain from phototropin, sterically blocking Rac1 interactions until
irradiation unwound a helix linking LOV to Rac1. Photoactivatable Rac1 (PA-Rac1)
could be reversibly and repeatedly activated using 458- or 473-nm light to
generate precisely localized cell protrusions and ruffling. Localized Rac
activation or inactivation was sufficient to produce cell motility and control
the direction of cell movement. Myosin was involved in Rac control of
directionality but not in Rac-induced protrusion, whereas PAK was required for
Rac-induced protrusion. PA-Rac1 was used to elucidate Rac regulation of RhoA in
cell motility. Rac and Rho coordinate cytoskeletal behaviours with seconds and
submicrometre precision. A mCherry reporter was then cloned at N-terminal of
PA-Rac1 to generate mCherry-PA-Rac1 construct which was then packaged to
adenovirus. Ad-CMV-mCherry-PA-Rac1 can be used to infect most of mammalian
cell types for time-lapse visualization of Rac1 activation via photoactivation.
Titer: 1x1010 ~ 1x1011 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
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