Product Category: human Adenovirus
Type5 (dE1/E3), mCherry-PA-Rac1-T17N adenovirus for over-expression of
mCherry-PA-Rac1 mutant (T17N) under CMV promoter; Ad-CMV-mCherry-PA-Rac1-T17N;
Ad-mCherry-PA-Rac1-T17N
Description: Rac1 is a key GTPase regulating actin cytoskeletal dynamics in
metazoan cells. Rac1 mutants were fused to the photoreactive LOV (light oxygen
voltage) domain from phototropin, sterically blocking Rac1 interactions until
irradiation unwound a helix linking LOV to Rac1. Photoactivatable Rac1 (PA-Rac1)
could be reversibly and repeatedly activated using 458- or 473-nm light to
generate precisely localized cell protrusions and ruffling. A mutation at T17N
abolished this effect. Ad-CMV-mCherry-PA-Rac1-T17N will over-express
mCherry-PA-Rac1 (T17N) in most of mammalian cell types in which Rac activation
won't happen thru photoactivation, serving as a control for Ad-CMV-mCherry-PA-Rac1.
Titer: 1x1010 ~ 1x1011 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
User Manual:
Shuttle Vector Map:
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