LV-hSyn-hM4D(Gi)-mCherry

Product Category: Pre-made recombinant lentivirus; LV-hSyn-hM4D(Gi)-mCherry; Syn-hM4D(Gi)-mCherry Lentivirus

Description: LV-hSyn-hM4D(Gi)-mCherry is a pre-made lentivirus which expresses Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter. The transgene was modified form of human Gi-coupled M4 muscarinic receptor, belonging to a class of chemogenetic receptors called designer receptors exclusively activated by designer drugs (DREADD) which allows engagement of the Gi signaling pathway in the presence of the synthetic ligand clozapine-N-oxide (CNO), leading to intracellular calcium ion release and inhibit neuronal excitation.  Ready to use format. 

Titer: > 1x10⁹ TU/mL

Selection Antibiotic: Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 

Transfer Vector Map:


* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm