Product Category: Pre-made
recombinant lentivirus; LV-Syn-hM3D(Gq)-mCherry; Syn-hM3D(Gq)-mCherry
Lentivirus
Description: LV-Syn-hM3D(Gq)-mCherry
is a
pre-made lentivirus which expresses Gq-coupled hM3D DREADD fused with
mCherry under the control of human synapsin promoter.
The transgene was
modified form of human Gq-coupled M3 muscarinic
receptor, belonging to a class of chemogenetic receptors called designer
receptors exclusively activated by designer drugs (DREADD) which allows
engagement of the Gq
signaling pathway in the presence of the synthetic ligand clozapine-N-oxide (CNO),
leading to intracellular calcium ion release and enhanced neuronal excitation.
Ready to
use format.
Titer: >
1x109 TU/mL
Selection Antibiotic: Puromycin
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2
x 25 µL (Std
Pack)
Storage Buffer: PBS
Infection Protocol:
Transfer Vector Map:
* Concentrated
via PEG precipitation followed by
ultra-centrifugation at 25000 rpm