AAV-CMV-FLPo (AAV Serotype 5)

Price: $488.00

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  • Catalog #: SL101457
  • Unit Size: 1 x >30 L (>1E+13 VG/mL)

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Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-CBA-FLPo; AAV5-CMV-FLPo; AAV5-CBA-FLPo

Product Category: Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-FLPo; AAV5-CMV-FLPo; AAV5-FLPo

Description: FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the ȫ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.  

FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. 
This AAV5
-CMV-FLPo pre-packaged rAAV in serotype 5 (with capsid from AAV serotype 5 and 2xITR from AAV serotype 2) will over-express FLPo recombinase under CMV promoter.  Ready to use format.

Titer: > 1x10^13 VG/mL

Size: > 30 L (Std Pack)

Storage Buffer: Sterile PBS

Infection Protocol: 

Vector Map: 

Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-CBA-FLPo; AAV5-CMV-FLPo; AAV5-CBA-FLPo