Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-CBA-FLPo; AAV2-CMV-FLPo; AAV2-CBA-FLPo
Product
Category: Pre-made recombinant
AAV; AAV-CMV-FLPo; AAV-FLPo; AAV2-CMV-FLPo; AAV2-FLPo
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the Þ integrase family and recognizes distinct 34 bp FRT
sequence. It has been widely used for generating conditional knockout mice,
mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed
inefficient recombinase activity due to thermo-instability of the FLP protein.
Subsequent screening for thermo-stable mutants resulted in the identification of
FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the
recombination efficiency of FLPe in cells remains very low because of its
non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse. This
AAV2-CMV-FLPo
pre-packaged rAAV in serotype 2 (with capsid from AAV serotype 2 and 2xITR from AAV
serotype 2) will over-express
FLPo recombinase under
CMV promoter. Ready
to use format.
Titer: >
1x10^13 VG/mL
Size: > 30 µL
(Std Pack)
Storage Buffer: Sterile PBS
Infection Protocol: 
Vector Map:
