Description
GenJet™ DNA In Vitro
Transfection Reagent for MCF 10A Cell is pre-optimized for transfecting the
non-tumorigenic epithelial
cell line,
MCF 10A.
The MCF 10A cell line is a
non-tumorigenic epithelial cell line. The cells are positive for epithelial
sialomucins, cytokeratins and milk fat globule antigen. They exhibit three
dimensional growth in collagen, and form domes in confluent cultures. Thus far,
the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and
epidermal growth factor (EGF). By electron microscopy the cells display
characteristics of luminal ductal cells but not of myoepithelial cells.
With our proprietary
transfection booster conjugation technology, this reagent was formulated to transfect
MCF 10A cells
with up to 50% efficiency (GFP positive cells).
|
Summary of
Optimal Shaved Cell Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of shaving
Cell density for plating
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Incubation time of shaved cells with transfection complex
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
DMEM/F12
with 5% HS and other additives
95~100%
1.3x10^5 cells per square centimeter
4:1
Serum-free DMEM/F12
15 minutes at RT
20 minutes at 37 degree
48 hours
EGFP
pEGFP-N3 (CMV promoter)
50% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
To request a free trial sample, please
Create An Account with us to enter your shipping address and email
us at
order@signagen.com
