Description
GenJet™ DNA In Vitro
Transfection Reagent for Jurkat cell is pre-optimized for transfecting Jurkat cells. Refer to the following optimal transfection conditions for
maximal transfection efficiency on Jurkat cells. GenJet™ reagent, 1.0 ml, is
sufficient for 300 to 600 transfections in 24 well plates or 50 to 100
transfections in 6 well plates.
|
Summary
of Optimal Transfection Conditions:
Confluence on
the day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~70%
PRMI with 10% FBS
3:1
1xGenJet™ Transfection Buffer
15 minutes at RT
Yes
No
48 hours
EGFP
pEGFP-N3 (CMV promoter)
80% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet & Protocol
