Pre-made human adenovirus serotype 5 (dE1/E3), iCre recombinase adenovirus with puromycin selection co-expression via IRES; Ad-CMV-iCre-IRES-Puro; Ad-CMV-iCre-Puro
Product Category: Pre-made human
adenovirus serotype 5 (dE1/E3), iCre recombinase adenovirus; Ad-CMV-iCre; Ad-iCre
Description: Cre
recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the
site-specific recombination of DNA between loxP sites. Cre recombinase is used
as a tool to genetically modify genes, such as to delete a segment of DNA
flanked by LoxP sites in cells or experimental animals. By optimizing the
mammalian codon usage for Cre recombinase, Cre expression is improved in the
mammalian cells. This codon-improved Cre (known as iCre) transgene shows lower
CpG content from the prokaryotic coding sequence, thus reducing the chances of
epigenetic silencing in mammals.
Ad-CMV-iCre is a pre-packaged adenovirus which over-expresses iCre
recombinase under CMV promoter. This product used in the Cre-lox system as a genetic tool to generate site-specific recombination of DNA between loxP sites in cultured cells and
animal experiments. Ready to use format.
Titer: 1x1010 ~ 1x1011
PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
User Manual:
Shuttle Vector Map:
