Pre-made recombinant lentivirus; LV-CaMKII-GCaMP6f; CaMKII-GCaMP6f Lentivirus
Product Category: Pre-made recombinant lentivirus; LV-CaMKII-GCaMP6f; CaMKII-GCaMP6f Lentivirus
Description:
GCaMP
is a genetically encoded calcium indicator (GECI) and created from a fusion of
green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence
from myosin light chain kinase. When GECI
binds Ca2+, the conformational change of GCaMP induces proximity of the GFP
parts, eliciting strong GFP fluorescence signal, which allows measuring action
potentials and other receptor activation events that trigger Ca2+ influx. The
advantage of GECI's are that they can be genetically specified for studies in
living organisms.
GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other
sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level.
Mutations in calmodulin part of GCaMP6 created several variants which showed
different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and
medium - GCaMP6m).
LV-CaMKII-GCaMP6f is pre-made lentivirus which expresses
GCaMP6f under CaMKII promoter
for neuronal exclusive expression. Ready to use format.
Titer: > 1x109 TU/mL
Selection Antibiotic: None
Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection
Size: 2 x 25 µL (Std Pack)
Storage Buffer: PBS
Infection Protocol: 
Transfer Vector Map:
* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm
