GenJet™ In Vitro DNA Transfection Reagent for Epithelial Cell

Price: $159.00

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  • Catalog #: SL100489-EPI
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for epithelial cell is pre-optimized for epithelial cells. By cross-linking a peptide ligand, this pre-optimized GenJet™ reagent specifically binds epithelial cells and give superior efficiency on epithelial cells. 

Refer to the following optimal transfection conditions for maximal transfection efficiency on epithelial cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter)

65%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer



A comparison showing superior efficiency of the pre-optimized GenJet (Ver. II) on normal human colonic epithelial cells.  Transfection was performed per the vendors' protocol.  0.5 µg of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human colonic epithelial cells with different transfection methods.  The GFP positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope 24 hours post transfection.


Data Sheet




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