GenJet™ In Vitro DNA Transfection Reagent for L929 Cell

Price: $168.00

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  • Catalog #: SL100489-L929
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for L929 cell is pre-optimized for transfecting L929 cells. L929 is a murine aneuploid fibrosarcoma cell line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF initiates apoptosis and subsequent cell death.  The level of detection is on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold more sensitive is WEHI-164. Some L929 subclones have been developed that allow detection of TNF at concentrations of approximately 30 pmol/L (approximately 500 fg/mL). Sublines that are resistant to TNF have been established also.

Refer to the following optimal transfection conditions for maximal transfection efficiency on L929 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter)

65%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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