Primers: 5’-AGTCCGCCCTGAGCAAAGA-3’ (eGFP-F) 5’-TCCAGCAGGACCATGTGATC-3’ (eGFP-R) Probe: CCCAACGAGAAGCG
Miscellaneous - 4. page
Validated Target Region against Mouse Lamtor1
CGTATGCCTATAGTGCACTTT
Two Validated Target Regions against Mouse ROCK2
CAGAAGCGTTGTCTTATGCAA TTGGATAAACATGGACATCTA
Two validated target regions
N-Taf : CTCAGGCTAAGCCTCCTGATT C/N-Taf: TGGAGGAAGCAGAATTAGAAA
Validated Target Region against Mouse Rock2
5’-CAGAAGCGTTGTCTTATGCAA-3’ 5’-TTGGATAAACATGGACATCTA-3’
Human VMD2 Promoter
AATTC TGTCATTTTA CTAGGGTGAT GAAATTCCCA AGCAACACCATCCTTTTCAG ATAAGG GCAC TGAGGCTGAG AGAGGAGCTG AAACCTACCC GGCGTCACCACACACAGGTG GCAAG GCTGG GACCAGAAAC CAGGACTGTT GACTGCAGCC CGGTATTCATTCTTTCCATA GCCCA CAGGG CTGTCAAAGA CCCCAGGGCC TAGTCAGAGG CTCCTCCTTCCTGGAGAGTT CCTGG CACAG AAGTTGAAGC TCAGCACAGC CCCCTAACCC CCAACTCTCTCTGCAAGGCC TCAGG […]
Validated Target Region against Mouse TIMP1 Gene
# 1. tgcacagtgtttccctgtt # 2. tcaagatgactaagatgct # 3. tgcacagtgtttccctgtt
Determining the concentration of protein in SDS-PAGE gel bands using ImageJ
Determining the concentration of protein in SDS-PAGE gel bands using ImageJ To determine protein concentration you will need to have a standard curve to compare your samples to – For […]
Commonly Used Primers
3’AOX1 GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer 5’AOX1 GACTGGTTCCAATTGACAAGC For Pichia vectors with AOX1 promoter, forward primer 35S promoter CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer AC5 ACACAAAGCCGCTCCATCAG […]
Sim1 Specific Promoter for PVN Neurons
1 GGTACCGAGG CGCGCAGCCG TGGGGAAATC CTGGCCACTG TCTGGGATGC 51 TATTCGCACG CGTTCCTGGA GCCTCGGCTA GCCAGCCCCC GCAAGGCCGC 101 ACGCTGTGCG CAGACACCCG AGTCTAGGGA GCGCGAGTGT CACTCGGCAC 151 GCCGAAGACC TCGGGGTTTG GAGCGGCGGC GGCAGCGGGG CAGCGGCGAC 201 CCCGCGCCCG GGGCTCATTA GGAGCGGAGC TGCCAGGCTC ACTGTAGGAC […]