LV-EF1α-tdTOMATO-IRES-Puro

Price: $468.00

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  • Catalog #: SL100323
  • Unit Size: 2 x 25 µL (>10^9 TU/mL)

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Pre-made recombinant lentivirus; LV-EF1α-tdTOMATO-IRES-Puro; LV-EF1A-tdTOMATO-Puro; EF1α-tdTOMATO-Puro Lentivirus; EF1A-tdTOMATO-Puro Lentivirus

Product Category: Pre-made recombinant lentivirus; LV-EF1α-tdTOMATO-IRES-Puro; LV-EF1A-tdTOMATO-Puro; EF1α-tdTOMATO-Puro Lentivirus; EF1A-tdTOMATO-Puro Lentivirus

Description: LV-EF1α-tdTOMATO-IRES-Puro is a pre-made lentivirus which expresses tdTOMATO under EF1α promoter with co-expression of puromycin selection via IRES. tdTOMATO, tandem dimeric (pseudo-monomeric) derivative of DsRed, is an exceptionally bright red fluorescent protein with around 6 times brighter than EGFP.  Because the two subunits of tdTomato are linked covalently, it behaves as a monomer, and has been used successfully for N- and C- terminal fusions. It shows very low aggregation, excellent photostability and its half-time for maturation is just one hour at 37°C. The red emission and brightness of tdTomato’s make it ideal for both in vitro and in vivo imaging studies.

Titer: > 1x109 TU/mL

Selection Antibiotic:
Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol:


Transfer Vector Map:
Pre-made recombinant lentivirus; LV-EF1α-tdTOMATO-IRES-Puro; LV-EF1A-tdTOMATO-Puro; EF1α-tdTOMATO-Puro Lentivirus; EF1A-tdTOMATO-Puro Lentivirus


*
Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm




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