shRNA Validation Service via Cell-based Reporter System
shRNA Validation Service
via Cell-based Reporter System:
- Cloning ORF of gene of interests (GOI) into an adenovirus shuttle vector with a reporter.
- Design and synthesize ~6 shRNA oligoes followed by cloning them to the above
shuttle
construct to obtain shRNA validation plasmid.
- Transient transfection the shRNA validation plasmid.
- Quantification of target gene silencing via FACS (for GFP expression) or
luminometer (for luciferase expression).
Available shRNA Validation Platforms:
- Constitutive shRNA knockdown
- Inducible shRNA knockdown
Guaranteed Knockdown:
We guarantee at least 1 shRNA at >80% knockdown of mRNA level in the tested cells by
screening ~6 shRNA oligoes.
We offer discount for
new customer, please
request a quote with us today.
Figure 1. A Cartoon Showing shRNA
Validation Process for shRNA Adenovirus Production.
