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Quantification of AAV particles and empty capsids by optical density measurement

Predicted relationship of capsid to vector genome ratio (cp/vg) and absorbance ratio (A260/A280) for a denatured rAAV*. The theoretical A260/A280 ratio was plotted for the rAAV vector with a cp/vg ratio ranging from 1 (no empty capsids) to 50 (98% empty capsids).

* rAAV was denatured in conditions of 0.1% SDS and 10 min at 75°C prior to absorbance measurement.

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