| Dye | Structure | Color | Vendor | Cat # | Price | Features |
| SYBR Green 1 | Green | unstable at 4 °C | ||||
| SYTO-82 | Orange | does not inhibit PCR, show no preferential binding to GC rich sequences and does not influence melting temperature, Tm, even at high concentrations | ||||
| SYTO-13 | Green | does not inhibit PCR, show no preferential binding to GC rich sequences and does not influence melting temperature, Tm, even at high concentrations | ||||
| SYTO-9 | Green | produces highly reproducible DNA melting curves over a broader range of dye concentrations, low PCR inhibition | ||||
| SYTO-16 | melting curve analysis: produces the sharpest peaks. | |||||
| SYTO-80 | Orange | |||||
| EvaGreen | Green | melting curve analysis: produces the sharpest peaks. | ||||
| BEBO | 468/492 | TATAA BiocenterChalmers University of Technology | ||||
| BOXTO | 515/552 | TATAA BiocenterChalmers University of Technology | ||||
| BETIBO | 468/492 | |||||
| BOXTO-MEE | Chalmers University of Technology | better stability, 45 days storage at 4 °C. better than BOXTO, BOXTO-PRO, BEBO, BETIBO and SYBR Green 1 |
| Dye | Concentration | R2 | Slope | Efficiency |
| SYBR Green | 0.32 × (0.64 μM) | 0.985 | -3.40 | 97% |
| SYBR Green | 1× (2 μM) | 0.395 | -2.13 | 195% |
| EvaGreen | 1× | 0.995 | -3.30 | 101% |
| EvaGreen | 5× | 0.999 | -3.46 | 95% |
| SYTO 13 | 5 μM | 0.993 | -3.42 | 96% |
| SYTO 13 | 10 μM | 0.993 | -3.44 | 95% |
| SYTO-16 | 5 μM | 0.983 | -3.30 | 101% |
| SYTO-16 | 10 μM | 0.992 | -3.43 | 96% |
| SYTO 80 | 10 μM | 0.992 | -3.42 | 96% |
| SYTO 80 | 20 μM | 0.986 | -3.41 | 96% |
| SYTO 82 | 10 μM | 0.998 | -3.41 | 96% |
| SYTO 82 | 20 μM | 0.996 | -3.37 | 98% |