Transfection Reagent Selection Chart.
Reagents | Features | Transfection Type | Toxicity* |
CalFectin™ | – Traditional recipe with new formulation – Most affordable and efficient – Best for large scale virus, antibody and protein production |
DNA (Yes) siRNA (No) DNA/siRNA (No) |
++++ |
GenJet™ | – Low toxicity for very sensitive cells – Maximum cell viability |
DNA (Yes) siRNA (No) DNA/siRNA (No) |
++ |
GenJet™ (Ver. II) | – 100% DNA condensing groups released – Efficient for commonly used cell types |
DNA (Yes) siRNA (No) DNA/siRNA (No) |
+++ |
GenJet™ Plus | – Enhanced conjugate for maximal efficiency – Extremely good for very long DNAs |
DNA (Yes) siRNA (No) DNA/siRNA (No) |
++ |
LipoD293™ | – Enhanced conjugate with liposome for maximal efficiency – Best for lentivirus, antibody and protein production |
DNA (Yes) siRNA (No) DNA/siRNA (No) |
+++ |
LipoJet™ | – Fluorinated cationic lipids – Best transfection efficiency with least toxicity |
DNA (Yes) siRNA (Yes) DNA/siRNA (Yes) |
+ |
PolyJet™ | – Biodegradable polycationic polymer – Excellent efficiency for common used mammalian cells |
DNA (Yes) siRNA (No) DNA/siRNA (No) |
+++ |
* The cell viability was evaluated by MTT assay. Total 0.5 µg of pEGFP-C1 was transfected to Hela and HEK293T cells respectively on 24-well plate with different transfection reagents in comparison of Lipofectaminne 2000 (toxicity arbitrary grade ++++) and Fugene 6 (toxicity arbitrary grade +)