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SignaGen’s In Vitro DNA Transfection Reagents

Transfection Reagent Selection Chart.

Reagents Features Transfection Type Toxicity*
CalFectin™ – Traditional recipe with new formulation
– Most affordable and efficient
– Best for large scale virus, antibody and protein production
DNA (Yes)
siRNA (No)
DNA/siRNA (No)
++++
GenJet™ – Low toxicity for very sensitive cells
– Maximum cell viability
DNA (Yes)
siRNA (No)
DNA/siRNA (No)
++
GenJet™ (Ver. II) – 100% DNA condensing groups released
– Efficient for commonly used cell types
DNA (Yes)
siRNA (No)
DNA/siRNA (No)
+++
GenJet™ Plus – Enhanced conjugate for maximal efficiency
– Extremely good for very long DNAs
DNA (Yes)
siRNA (No)
DNA/siRNA (No)
++
LipoD293™ – Enhanced conjugate with liposome for maximal efficiency
– Best for lentivirus, antibody and protein production
DNA (Yes)
siRNA (No)
DNA/siRNA (No)
+++
LipoJet™ – Fluorinated cationic lipids
– Best transfection efficiency with least toxicity
DNA (Yes)
siRNA (Yes)
DNA/siRNA (Yes)
+
PolyJet™ – Biodegradable polycationic polymer
– Excellent efficiency for common used mammalian cells
DNA (Yes)
siRNA (No)
DNA/siRNA (No)
+++

* The cell viability was evaluated by MTT assay. Total 0.5 µg of pEGFP-C1 was transfected to Hela and HEK293T cells respectively on 24-well plate with different transfection reagents in comparison of Lipofectaminne 2000 (toxicity arbitrary grade  ++++) and Fugene 6 (toxicity arbitrary grade +)

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