Pre-made Adenovirus, Ad-CMV-Cas9-GFP from SignaGen
Product Category: Human Adenovirus Type5 (dE1/E3); Ad-CMV-Cas9-GFP
Description:
This recombinant human adenovirus type 5 expresses a codon-optimized
Cas9 nuclease fusion with GFP as reporter under CMV promote and can be used along with guided RNA for
genome-editing.
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted
genome engineering. Cas9 (CRISPR associated protein 9) is an RNA-guided
DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity
system. The cas9 nucleases
derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas
systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific
manner. With its ease in designing guide sequences to target specific genomic
loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to
TALEN and Zinc finger nuclease platforms.
Titer: 1x1010 ~ 1x1011 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
User Manual:
Shuttle Vector Map: