Price: $618.00

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  • Catalog #: SL101107
  • Unit Size: 1 x 200 µL

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Pre-made Adenovirus, Ad-CMV-Cas9-GFP from SignaGen

Product Category: Human Adenovirus Type5 (dE1/E3); Ad-CMV-Cas9-GFP

This recombinant human adenovirus type 5 expresses a codon-optimized Cas9 nuclease fusion with GFP as reporter under CMV promote and can be used along with guided RNA for genome-editing.

The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering.  Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity system.  The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.

Titer: 1x1010 ~ 1x1011 PFU/mL

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

User Manual:

Shuttle Vector Map:
Ad-CMV-Cas9-GFP; Ad-Cas9-GFP; Pre-made Adenovirus

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