LV-UBC-mCherry-GFP-LC3

$468.00

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  • Catalog #: SL100387
  • Unit Size: 2 x 25 µL (>10^9 TU/mL)

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Pre-made recombinant lentivirus | LV-UBC-mCherry-GFP-LC3 | UBC-mCherry-GFP-LC3 Lentivirus Pre-made recombinant lentivirus | LV-GFAP-iCre-P2A-GFP | GFAP-iCre-P2A-GFP Lentivirus

Product Category: Pre-made recombinant lentivirus | LV-UBC-mCherry-GFP-LC3 | UBC-mCherry-GFP-LC3 Lentivirus

Description: 
LV-UBC-mCherry-GFP-LC3 is a pre-made lentiviral vector that overexpresses an mCherry-GFP-LC3 fusion protein under control of the UBC promoter in mammalian cells. This vector enables visualization of autophagic flux: free autophagosomes display both GFP and mCherry fluorescence, whereas autophagosomes fused with lysosomes (autolysosomes) show mCherry fluorescence only, due to the acid sensitivity and quenching of GFP. Ready to use format.

Titer: > 1x10TU/mL

Selection Antibiotic: Puro


Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 


Transfer Vector Map:
Pre-made recombinant lentivirus | LV-UBC-mCherry-GFP-LC3 | UBC-mCherry-GFP-LC3 Lentivirus

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm




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