LV-Syn-DIO-GFP

Product Category: Pre-made recombinant lentivirus; LV-Syn-DIO-GFP; DIO-GFP lentivirus

Description:
LV-Syn-DIO-GFP is a pre-made lentivirus which will Cre dependently over-express GFP driven under human synapsin promoter as illustrated as the cartoon below. 

GFP is a green monomer which matures extremely bright, making it possible to see results very soon after activating transcription. It is highly photostable and resistant to photobleaching. In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5' promoter and is flanked by oppositely oriented loxP and lox2272 sites.  In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be "Flip-exchanged" or FLEXed, leading to the expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV). 



Titer: > 1x10⁹ TU/mL

Selection Antibiotic: None

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 

Transfer Vector Map:

 

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm