Product Category: Pre-made recombinant lentivirus; LV-EF1α-Cre-GFP-Puro; LV-EF1α-Cre-GFP-IRES-Puro; LV-EF1A-Cre-GFP-Puro; LV-EF1A-Cre-GFP-IRES-Puro; EF1A-Cre-GFP Lentivirus; EF1α-Cre-GFP Lentivirus
Description: Cre recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.
This pre-made lentivirus LV-EF1α-Cre-GFP-Puro expresses Cre recombinase fusion with GFP with co-expression of puromycin resistance in an IRES expression cassette under EF1α promoter. Ready to use format.
Titer: > 1x109 TU/mL
Selection Antibiotic: Puromycin
Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection
Size: 2 x 25 µL (Std Pack)
Storage Buffer: PBS
Infection Protocol:
Transfer Vector Map:
* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm